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Previous Volume 348:1764-1775 May 1, 2003 Number 18 Next

	Full Text PDF PDA Full Text PowerPoint Slide Set Editorial by Rai, K. R. Add to Personal Archive Add to Citation Manager Notify a Friend E-mail When Cited E-mail When Letters Appear Related Article by Murashige, N. PubMed Citation

ABSTRACT

Background The mutational status of immunoglobulin heavy-chain variable-region (IgV_H) genes in the leukemic cells of chronic lymphocytic leukemia (CLL) is an important prognostic factor in the disease. We investigated whether the expression of ZAP-70 by CLL cells correlated with the IgV_H mutational status, disease progression, and survival.

Methods The expression of ZAP-70 was analyzed in T-cell and B-cell lines and in peripheral-blood samples from 56 patients with CLL with the use of flow cytometry, Western blotting, and immunohistochemistry. The results were correlated with the IgV_H mutational status and clinical outcome.

Results ZAP-70 was detected by flow-cytometric analysis in cells of T-cell lineage and in leukemic cells from 32 of 56 patients with CLL. In all patients in whom at least 20 percent of the leukemic cells were positive for ZAP-70, IgV_H was unmutated, whereas IgV_H mutations were found in 21 of 24 patients in whom less than 20 percent of the leukemic cells were positive for ZAP-70 (P<0.001). Concordant results were obtained when ZAP-70 expression was assessed by immunohistochemistry or Western blotting. The level of ZAP-70 expression did not change over time (median, 37 months) in sequential samples from 30 patients with CLL. Patients with Binet stage A CLL who had at least 20 percent ZAP-70–positive leukemic cells had more rapid progression and poorer survival than those with less than 20 percent ZAP-70–positive cells.

Conclusions Among patients with CLL, expression of ZAP-70, as detected by flow-cytometric analysis, correlated with IgV_H mutational status, disease progression, and survival.

Source Information

From the Department of Hematology (M.C., F.B., A.L.-G., E.M.) and the Hematopathology Unit (N.V., B.B., D.C., M.R., S.M., E.C.), Institut d'Investigacions Biomèdiques August Pi i Sunyer, Hospital Clínic, Barcelona, Spain.

Ms. Crespo and Drs. Bosch and Villamor contributed equally to the article.

Address reprint requests to Dr. Bosch at the Department of Hematology, Hospital Clínic, Villarroel 170, Barcelona 08036, Spain, or at fbosch{at}clinic.ub.es.

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• Motiwala, T., Majumder, S., Kutay, H., Smith, D. S., Neuberg, D. S., Lucas, D. M., Byrd, J. C., Grever, M., Jacob, S. T. (2007). Methylation and Silencing of Protein Tyrosine Phosphatase Receptor Type O in Chronic Lymphocytic Leukemia. Clin. Cancer Res. 13: 3174-3181 [Abstract] [Full Text]  
• Sabattini, E., Orduz, R., Campidelli, C., Zinzani, P. L., Callea, V., Zupo, S., Cutrona, G., Morabito, F., Ferrarini, M., Pileri, S. (2007). B cell chronic lymphocytic leukaemia/small lymphocytic lymphoma: role of ZAP70 determination on bone marrow biopsy specimens. J. Clin. Pathol. 60: 627-632 [Abstract] [Full Text]  
• Josefsson, P., Geisler, C. H., Leffers, H., Petersen, J. H., Andersen, M. K., Jurlander, J., Buhl, A. M. (2007). CLLU1 expression analysis adds prognostic information to risk prediction in chronic lymphocytic leukemia. Blood 109: 4973-4979 [Abstract] [Full Text]  
• Wierda, W. G., O'Brien, S., Wang, X., Faderl, S., Ferrajoli, A., Do, K.-A., Cortes, J., Thomas, D., Garcia-Manero, G., Koller, C., Beran, M., Giles, F., Ravandi, F., Lerner, S., Kantarjian, H., Keating, M. (2007). Prognostic nomogram and index for overall survival in previously untreated patients with chronic lymphocytic leukemia. Blood 109: 4679-4685 [Abstract] [Full Text]  
• Fulci, V., Chiaretti, S., Goldoni, M., Azzalin, G., Carucci, N., Tavolaro, S., Castellano, L., Magrelli, A., Citarella, F., Messina, M., Maggio, R., Peragine, N., Santangelo, S., Mauro, F. R., Landgraf, P., Tuschl, T., Weir, D. B., Chien, M., Russo, J. J., Ju, J., Sheridan, R., Sander, C., Zavolan, M., Guarini, A., Foa, R., Macino, G. (2007). Quantitative technologies establish a novel microRNA profile of chronic lymphocytic leukemia. Blood 109: 4944-4951 [Abstract] [Full Text]  
• Ian Mockridge, C., Potter, K. N., Wheatley, I., Neville, L. A., Packham, G., Stevenson, F. K. (2007). Reversible anergy of sIgM-mediated signaling in the two subsets of CLL defined by VH-gene mutational status. Blood 109: 4424-4431 [Abstract] [Full Text]  
• Vallat, L. D., Park, Y., Li, C., Gribben, J. G. (2007). Temporal genetic program following B-cell receptor cross-linking: altered balance between proliferation and death in healthy and malignant B cells. Blood 109: 3989-3997 [Abstract] [Full Text]  
• Muntanola, A., Bosch, F., Arguis, P., Arellano-Rodrigo, E., Ayuso, C., Gine, E., Crespo, M., Abrisqueta, P., Moreno, C., Cobo, F., Lopez-Guillermo, A., Montserrat, E. (2007). Abdominal Computed Tomography Predicts Progression in Patients With Rai Stage 0 Chronic Lymphocytic Leukemia. JCO 25: 1576-1580 [Abstract] [Full Text]  
• Nowakowski, G. S., Hoyer, J. D., Shanafelt, T. D., Geyer, S. M., LaPlant, B. R., Call, T. G., Jelinek, D. F., Zent, C. S., Kay, N. E. (2007). Using Smudge Cells on Routine Blood Smears to Predict Clinical Outcome in Chronic Lymphocytic Leukemia: A Universally Available Prognostic Test. Mayo Clin Proc. 82: 449-453 [Abstract] [Full Text]  
• Bomben, R., Dal Bo, M., Capello, D., Benedetti, D., Marconi, D., Zucchetto, A., Forconi, F., Maffei, R., Ghia, E. M., Laurenti, L., Bulian, P., Del Principe, M. I., Palermo, G., Thorselius, M., Degan, M., Campanini, R., Guarini, A., Del Poeta, G., Rosenquist, R., Efremov, D. G., Marasca, R., Foa, R., Gaidano, G., Gattei, V. (2007). Comprehensive characterization of IGHV3-21-expressing B-cell chronic lymphocytic leukemia: an Italian multicenter study. Blood 109: 2989-2998 [Abstract] [Full Text]  
• O'Brien, S., Moore, J. O., Boyd, T. E., Larratt, L. M., Skotnicki, A., Koziner, B., Chanan-Khan, A. A., Seymour, J. F., Bociek, R. G., Pavletic, S., Rai, K. R. (2007). Randomized Phase III Trial of Fludarabine Plus Cyclophosphamide With or Without Oblimersen Sodium (Bcl-2 antisense) in Patients With Relapsed or Refractory Chronic Lymphocytic Leukemia. JCO 25: 1114-1120 [Abstract] [Full Text]  
• Grever, M. R., Lucas, D. M., Dewald, G. W., Neuberg, D. S., Reed, J. C., Kitada, S., Flinn, I. W., Tallman, M. S., Appelbaum, F. R., Larson, R. A., Paietta, E., Jelinek, D. F., Gribben, J. G., Byrd, J. C. (2007). Comprehensive Assessment of Genetic and Molecular Features Predicting Outcome in Patients With Chronic Lymphocytic Leukemia: Results From the US Intergroup Phase III Trial E2997. JCO 25: 799-804 [Abstract] [Full Text]  
• Gobessi, S., Laurenti, L., Longo, P. G., Sica, S., Leone, G., Efremov, D. G. (2007). ZAP-70 enhances B-cell-receptor signaling despite absent or inefficient tyrosine kinase activation in chronic lymphocytic leukemia and lymphoma B cells. Blood 109: 2032-2039 [Abstract] [Full Text]  
• Van Bockstaele, F., Pede, V., Janssens, A., Callewaert, F., Offner, F., Verhasselt, B., Philippe, J. (2007). Lipoprotein Lipase mRNA Expression in Whole Blood Is a Prognostic Marker in B Cell Chronic Lymphocytic Leukemia. Clin. Chem. 53: 204-212 [Abstract] [Full Text]  
• Venugopal, P., Gregory, S. A. (2007). Lymphoproliferative disorders. ASH-SAP 2007: 265-297 [Full Text]  
• Chanan-Khan, A., Miller, K. C., Musial, L., Lawrence, D., Padmanabhan, S., Takeshita, K., Porter, C. W., Goodrich, D. W., Bernstein, Z. P., Wallace, P., Spaner, D., Mohr, A., Byrne, C., Hernandez-Ilizaliturri, F., Chrystal, C., Starostik, P., Czuczman, M. S. (2006). Clinical Efficacy of Lenalidomide in Patients With Relapsed or Refractory Chronic Lymphocytic Leukemia: Results of a Phase II Study. JCO 24: 5343-5349 [Abstract] [Full Text]  
• Coignet, J.-G., Padmanabhan, S., Manochakian, R., Miller, K. C., Wallace, P., Block, A., Chanan-Khan, A. A. (2006). Co-Expression of CD38 with Zap-70 Is Predictive of Treatment Intervention in Patients (pts) with Early Stage Chronic Lymphocytic Leukemia (CLL).. ASH ANNUAL MEETING ABSTRACTS 108: 4945-4945 [Abstract]  
• Leong, A S-Y, Leong, T Y-M (2006). Newer developments in immunohistology.. J. Clin. Pathol. 59: 1117-1126 [Abstract] [Full Text]  
• Tinhofer, I., Rubenzer, G., Holler, C., Hofstaetter, E., Stoecher, M., Egle, A., Steurer, M., Greil, R. (2006). Expression levels of CD38 in T cells predict course of disease in male patients with B-chronic lymphocytic leukemia. Blood 108: 2950-2956 [Abstract] [Full Text]  
• Shanafelt, T. D., Witzig, T. E., Fink, S. R., Jenkins, R. B., Paternoster, S. F., Smoley, S. A., Stockero, K. J., Nast, D. M., Flynn, H. C., Tschumper, R. C., Geyer, S., Zent, C. S., Call, T. G., Jelinek, D. F., Kay, N. E., Dewald, G. W. (2006). Prospective Evaluation of Clonal Evolution During Long-Term Follow-Up of Patients With Untreated Early-Stage Chronic Lymphocytic Leukemia. JCO 24: 4634-4641 [Abstract] [Full Text]  
• Frey, U. H., Nuckel, H., Sellmann, L., Siemer, D., Kuppers, R., Durig, J., Duhrsen, U., Siffert, W. (2006). The GNAS1 T393C Polymorphism Is Associated with Disease Progression and Survival in Chronic Lymphocytic Leukemia.. Clin. Cancer Res. 12: 5686-5692 [Abstract] [Full Text]  
• Shanafelt, T. D., Byrd, J. C., Call, T. G., Zent, C. S., Kay, N. E. (2006). Narrative review: initial management of newly diagnosed, early-stage chronic lymphocytic leukemia.. ANN INTERN MED 145: 435-447 [Abstract] [Full Text]  
• Falini, B., Martelli, M. P., Bolli, N., Bonasso, R., Ghia, E., Pallotta, M. T., Diverio, D., Nicoletti, I., Pacini, R., Tabarrini, A., Galletti, B. V., Mannucci, R., Roti, G., Rosati, R., Specchia, G., Liso, A., Tiacci, E., Alcalay, M., Luzi, L., Volorio, S., Bernard, L., Guarini, A., Amadori, S., Mandelli, F., Pane, F., Lo-Coco, F., Saglio, G., Pelicci, P.-G., Martelli, M. F., Mecucci, C., for the GIMEMA Acute Leukemia Working Party, (2006). Immunohistochemistry predicts nucleophosmin (NPM) mutations in acute myeloid leukemia. Blood 108: 1999-2005 [Abstract] [Full Text]  
• Deaglio, S., Vaisitti, T., Aydin, S., Ferrero, E., Malavasi, F. (2006). In-tandem insight from basic science combined with clinical research: CD38 as both marker and key component of the pathogenetic network underlying chronic lymphocytic leukemia. Blood 108: 1135-1144 [Abstract] [Full Text]  
• Lin, K., Glenn, M. A., Harris, R. J., Duckworth, A. D., Dennett, S., Cawley, J. C., Zuzel, M., Slupsky, J. R. (2006). c-Abl Expression in Chronic Lymphocytic Leukemia Cells: Clinical and Therapeutic Implications.. Cancer Res. 66: 7801-7809 [Abstract] [Full Text]  
• Yee, K. W. L., O'Brien, S. M. (2006). Chronic Lymphocytic Leukemia: Diagnosis and Treatment. Mayo Clin Proc. 81: 1105-1129 [Abstract] [Full Text]  
• Del Principe, M. I., Del Poeta, G., Buccisano, F., Maurillo, L., Venditti, A., Zucchetto, A., Marini, R., Niscola, P., Consalvo, M. A. I., Mazzone, C., Ottaviani, L., Panetta, P., Bruno, A., Bomben, R., Suppo, G., Degan, M., Gattei, V., de Fabritiis, P., Cantonetti, M., Lo Coco, F., Del Principe, D., Amadori, S. (2006). Clinical significance of ZAP-70 protein expression in B-cell chronic lymphocytic leukemia. Blood 108: 853-861 [Abstract] [Full Text]  
• Koller, C., Bekele, B. N., Zhou, X., Park, C., Estrov, Z., O'Brien, S., Keating, M., Jilani, I., Giles, F. J., Kantarjian, H. M., Albitar, M. (2006). Plasma thrombopoietin compared with immunoglobulin heavy-chain mutation status as a predictor of survival in chronic lymphocytic leukemia. Blood 108: 1001-1006 [Abstract] [Full Text]  
• Deglesne, P.-A., Chevallier, N., Letestu, R., Baran-Marszak, F., Beitar, T., Salanoubat, C., Sanhes, L., Nataf, J., Roger, C., Varin-Blank, N., Ajchenbaum-Cymbalista, F. (2006). Survival response to B-cell receptor ligation is restricted to progressive chronic lymphocytic leukemia cells irrespective of zap70 expression.. Cancer Res. 66: 7158-7166 [Abstract] [Full Text]  
• Krober, A., Dohner, H., Stilgenbauer, S. (2006). In Reply. JCO 24: 3219-3220 [Full Text]  
• Tsimberidou, A.-M., O'Brien, S., Khouri, I., Giles, F. J., Kantarjian, H. M., Champlin, R., Wen, S., Do, K.-A., Smith, S. C., Lerner, S., Freireich, E. J., Keating., M. J. (2006). Clinical Outcomes and Prognostic Factors in Patients With Richter's Syndrome Treated With Chemotherapy or Chemoimmunotherapy With or Without Stem-Cell Transplantation. JCO 24: 2343-2351 [Abstract] [Full Text]  
• Secchiero, P., Barbarotto, E., Tiribelli, M., Zerbinati, C., di Iasio, M. G., Gonelli, A., Cavazzini, F., Campioni, D., Fanin, R., Cuneo, A., Zauli, G. (2006). Functional integrity of the p53-mediated apoptotic pathway induced by the nongenotoxic agent nutlin-3 in B-cell chronic lymphocytic leukemia (B-CLL). Blood 107: 4122-4129 [Abstract] [Full Text]  
• Richardson, S. J., Matthews, C., Catherwood, M. A., Alexander, H. D., Carey, B. S., Farrugia, J., Gardiner, A., Mould, S., Oscier, D., Copplestone, J. A., Prentice, A. G. (2006). ZAP-70 expression is associated with enhanced ability to respond to migratory and survival signals in B-cell chronic lymphocytic leukemia (B-CLL). Blood 107: 3584-3592 [Abstract] [Full Text]